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The 2 protein bands from 40 kDa to 30 kDa ended up absent soon after 24 h incubation at 45 °C. For the rest of the bands, many concentrations of all 3 proteases retained protein bands akin to their unique molar masses (Figure 6a,b). The presence of bands at low molecular weight in fish roe right before hydrolysis was unanticipated. From the report